METATOOL OUTPUT Version 3.5 (28.03.2001) C:\METATOOL\META35~1.EXE INPUT FILE: EXAMPLE13.TXT INTERNAL METABOLITES: 19 REACTIONS: 6 3 external H2O 1 int 5,10-Methylenetetrahydrofolate 1 int glycine 1 int tetrahydrofolate 1 int L-serine 1 int (5-L-Glutamyl)-peptide 1 int an_amino_acid 1 int peptide 1 int 5-L-glutamyl_amino_acid 1 int beta-D-oligosaccarides 1 int n_D-glucose 1 int L-Asparagine 1 int L-aspartate 1 int NH3 1 int ATP 1 int L-Asp 1 int NH4+ 1 int AMP 1 int diphosphate 1 int L-Asn 20 metabolites, 22 is the summarized frequency edges frequency of nodes 0 0 1 19 2 0 3 1 STOICHIOMETRIC MATRIX matrix dimension r19 x c6 -1 0 0 0 0 0 -1 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 -1 0 0 0 0 0 -1 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 -1 0 0 0 0 0 1 0 0 0 0 0 0 -1 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 -1 0 0 0 0 0 -1 0 0 0 0 0 -1 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 1 The following line indicates reversible (0) and irreversible reactions (1) 0 1 1 1 1 1 rows and columns are sorted as declared in the inputfile NOT BALANCED INTERNAL METABOLITES 5,10-Methylenetetrahydrofolate glycine tetrahydrofolate L-serine (5-L-Glutamyl)-peptide an_amino_acid peptide 5-L-glutamyl_amino_acid beta-D-oligosaccarides n_D-glucose L-Asparagine L-aspartate NH3 ATP L-Asp NH4+ AMP diphosphate L-Asn KERNEL matrix dimension r1 x c6 0 1 0 0 0 0 6 reactions (columns) are sorted in the same order as in the ENZREV ENZIRREV section. enzymes 1: 2.1.2.1 irreversible overall reaction - not found - The remaining overall reactions perform no net transformation of external metabolites. SUBSETS OF REACTIONS matrix dimension r1 x c6 0 1 0 0 0 0 6 reactions (columns) are sorted in the same order as in the ENZREV ENZIRREV section. enzymes 1: 2.1.2.1 irreversible overall reaction - not found - The remaining overall reactions perform no net transformation of external metabolites. REDUCED SYSTEM with 19 branch point metabolites in 1 reactions (columns) matrix dimension r0 x c1 The following line indicates reversible (0) and irreversible reactions (1) 1 -> Branch metabolites are : met cons built reactions -> No branch metabolites are : met cons built reactions 5,10-Methylenetetrahydrofolate 1 0 1 r glycine 1 0 1 r tetrahydrofolate 0 1 1 r L-serine 0 1 1 r (5-L-Glutamyl)-peptide 1 0 1 i an_amino_acid 1 0 1 i peptide 0 1 1 i 5-L-glutamyl_amino_acid 0 1 1 i beta-D-oligosaccarides 1 0 1 i n_D-glucose 0 1 1 i L-Asparagine 1 0 1 i L-aspartate 0 1 1 i NH3 0 1 1 i ATP 1 0 1 i L-Asp 1 0 1 i NH4+ 1 0 1 i AMP 0 1 1 i diphosphate 0 1 1 i L-Asn 0 1 1 i CONVEX BASIS matrix dimension r1 x c6 0 1 0 0 0 0 enzymes 1: 2.1.2.1 irreversible overall reaction - not found - The remaining overall reactions perform no net transformation of external metabolites. CONSERVATION RELATIONS matrix dimension r14 x c19 1 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 1 : 5,10-Methylenetetrahydrofolate + glycine = const 2 : 5,10-Methylenetetrahydrofolate + tetrahydrofolate = const 3 : 5,10-Methylenetetrahydrofolate + L-serine = const 4 : (5-L-Glutamyl)-peptide + an_amino_acid = const 5 : (5-L-Glutamyl)-peptide + peptide = const 6 : (5-L-Glutamyl)-peptide + 5-L-glutamyl_amino_acid = const 7 : beta-D-oligosaccarides + n_D-glucose = const 8 : L-Asparagine + L-aspartate = const 9 : L-Asparagine + NH3 = const 10 : ATP + L-Asp = const 11 : ATP + NH4+ = const 12 : ATP + AMP = const 13 : ATP + diphosphate = const 14 : ATP + L-Asn = const ELEMENTARY MODES matrix dimension r1 x c6 0 1 0 0 0 0 6 reactions (columns) are sorted in the same order as in the ENZREV ENZIRREV section. enzymes 1: 2.1.2.1 irreversible overall reaction - not found - The remaining overall reactions perform no net transformation of external metabolites. The elementary modes ARE EQUAL to convex basis.