METATOOL OUTPUT Version 3.5 (28.03.2001) C:\METATOOL\META35~1.EXE INPUT FILE: EXAMPLE14.TXT INTERNAL METABOLITES: 13 REACTIONS: 5 2 external H2O 1 int 5,6,7,8-Tetrahydrofolate 1 int NADP+ 1 int 7,8-dihydrofolate 1 int NADPH 1 int An_orthophosphoric_monoester 1 int an_alcohol 1 int phosphate 1 int GTP 1 int formate 1 int 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)dihydropteridine_triphosphate 1 int 6-(L-erythro-1,2-Dihydroxypropyl_3-triphosphate)-7,8-dihydropterin 1 int 6-(1,2-dioxopropyl)-5,6,7,8-tetrahydropterin 1 int triphosphate 14 metabolites, 15 is the summarized frequency edges frequency of nodes 0 0 1 13 2 1 STOICHIOMETRIC MATRIX matrix dimension r13 x c5 -1 0 0 0 0 -1 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 -1 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 -1 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 -1 0 0 0 0 1 0 0 0 0 1 The following line indicates reversible (0) and irreversible reactions (1) 0 1 1 1 1 rows and columns are sorted as declared in the inputfile NOT BALANCED INTERNAL METABOLITES 5,6,7,8-Tetrahydrofolate NADP+ 7,8-dihydrofolate NADPH An_orthophosphoric_monoester an_alcohol phosphate GTP formate 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)dihydropteridine_triphosphate 6-(L-erythro-1,2-Dihydroxypropyl_3-triphosphate)-7,8-dihydropterin 6-(1,2-dioxopropyl)-5,6,7,8-tetrahydropterin triphosphate KERNEL matrix dimension r1 x c5 0 1 0 0 0 5 reactions (columns) are sorted in the same order as in the ENZREV ENZIRREV section. enzymes 1: 1.5.1.3 irreversible overall reaction - not found - The remaining overall reactions perform no net transformation of external metabolites. SUBSETS OF REACTIONS matrix dimension r1 x c5 0 1 0 0 0 5 reactions (columns) are sorted in the same order as in the ENZREV ENZIRREV section. enzymes 1: 1.5.1.3 irreversible overall reaction - not found - The remaining overall reactions perform no net transformation of external metabolites. REDUCED SYSTEM with 13 branch point metabolites in 1 reactions (columns) matrix dimension r0 x c1 The following line indicates reversible (0) and irreversible reactions (1) 1 -> Branch metabolites are : met cons built reactions -> No branch metabolites are : met cons built reactions 5,6,7,8-Tetrahydrofolate 1 0 1 r NADP+ 1 0 1 r 7,8-dihydrofolate 0 1 1 r NADPH 0 1 1 r An_orthophosphoric_monoester 1 0 1 i an_alcohol 0 1 1 i phosphate 0 1 1 i GTP 1 0 1 i formate 0 1 1 i 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)dihydropteridine_triphosphate 0 1 1 i 6-(L-erythro-1,2-Dihydroxypropyl_3-triphosphate)-7,8-dihydropterin 1 0 1 i 6-(1,2-dioxopropyl)-5,6,7,8-tetrahydropterin 0 1 1 i triphosphate 0 1 1 i CONVEX BASIS matrix dimension r1 x c5 0 1 0 0 0 enzymes 1: 1.5.1.3 irreversible overall reaction - not found - The remaining overall reactions perform no net transformation of external metabolites. CONSERVATION RELATIONS matrix dimension r9 x c13 1 -1 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 1 0 1 1 : 5,6,7,8-Tetrahydrofolate + NADP+ = const 2 : 5,6,7,8-Tetrahydrofolate + 7,8-dihydrofolate = const 3 : 5,6,7,8-Tetrahydrofolate + NADPH = const 4 : An_orthophosphoric_monoester + an_alcohol = const 5 : An_orthophosphoric_monoester + phosphate = const 6 : GTP + formate = const 7 : GTP + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)dihydropteridine_triphosphate = const 8 : 6-(L-erythro-1,2-Dihydroxypropyl_3-triphosphate)-7,8-dihydropterin + 6-(1,2-dioxopropyl)-5,6,7,8-tetrahydropterin = const 9 : 6-(L-erythro-1,2-Dihydroxypropyl_3-triphosphate)-7,8-dihydropterin + triphosphate = const ELEMENTARY MODES matrix dimension r1 x c5 0 1 0 0 0 5 reactions (columns) are sorted in the same order as in the ENZREV ENZIRREV section. enzymes 1: 1.5.1.3 irreversible overall reaction - not found - The remaining overall reactions perform no net transformation of external metabolites. The elementary modes ARE EQUAL to convex basis.