#ifdef _cplusplus extern "C" { #endif #include "genomicdb.h" /* Function: show_Hscore_GenomicDB(hs,ofp) * * Descrip: shows the Hscore by the GenomicDB information * * * * Arg: hs [UNKN ] High Score structure [Hscore *] * Arg: ofp [UNKN ] output file [FILE *] * */ # line 76 "genomicdb.dy" void show_Hscore_GenomicDB(Hscore * hs,FILE * ofp) { int i; for(i=0;ilen;i++) fprintf(ofp,"Query [%20s] Target [%20s] %d\n",hs->ds[i]->query->name,hs->ds[i]->target->name,hs->ds[i]->score); } /* Function: get_Genomic_from_GenomicDB(gendb,de) * * Descrip: Gets Genomic sequence out from * the GenomicDB using the information stored in * dataentry * * * Arg: gendb [UNKN ] Undocumented argument [GenomicDB *] * Arg: de [UNKN ] Undocumented argument [DataEntry *] * * Return [UNKN ] Undocumented return value [Genomic *] * */ # line 89 "genomicdb.dy" Genomic * get_Genomic_from_GenomicDB(GenomicDB * gendb,DataEntry * de) { Sequence * seq; Sequence * temp; /* we need to get out the Sequence from seqdb */ if( gendb == NULL || de == NULL ) { warn("Cannot get a genomic sequence from NULL objects. Ugh!"); return NULL; } if( gendb->is_single_seq) { if( de->is_reversed == TRUE ) return hard_link_Genomic(gendb->revsingle); else return hard_link_Genomic(gendb->single); } seq = get_Sequence_from_SequenceDB(gendb->sdb,de); if( seq == NULL ) { warn("Cannot get entry for %s from Genomic db",de->name); } /* check dna status. We assumme someone knows what he is doing when he makes a genomic db!*/ if( seq->type != SEQUENCE_DNA) { warn("Sequence from %s data entry doesn't look like DNA. Forcing it to",de->name); } force_to_dna_Sequence(seq,1.0,NULL); if( de->is_reversed == TRUE ) { temp = reverse_complement_Sequence(seq); free_Sequence(seq); seq = temp; } return Genomic_from_Sequence_Nheuristic(seq,gendb->length_of_N); } /* Function: dataentry_add_GenomicDB(de,cs,gendb) * * Descrip: adds information to dataentry from GenomicDB * * will eventually add file offset and format information * * * Arg: de [UNKN ] Undocumented argument [DataEntry *] * Arg: cs [UNKN ] Undocumented argument [ComplexSequence *] * Arg: gendb [UNKN ] Undocumented argument [GenomicDB *] * * Return [UNKN ] Undocumented return value [boolean] * */ # line 138 "genomicdb.dy" boolean dataentry_add_GenomicDB(DataEntry * de,ComplexSequence * cs,GenomicDB * gendb) { de->name = stringalloc(cs->seq->name); de->is_reversed = is_reversed_Sequence(cs->seq); if( gendb->is_single_seq ) { return TRUE; } add_SequenceDB_info_DataEntry(gendb->sdb,de); return TRUE; } /* Function: init_GenomicDB(gendb,return_status) * * Descrip: top level function which opens the Genomic database * * * Arg: gendb [UNKN ] protein database [GenomicDB *] * Arg: return_status [WRITE] the status of the open from database.h [int *] * * Return [UNKN ] Undocumented return value [ComplexSequence *] * */ # line 158 "genomicdb.dy" ComplexSequence * init_GenomicDB(GenomicDB * gendb,int * return_status) { ComplexSequence * cs; Sequence * seq; if( gendb->is_single_seq == TRUE) { *return_status = DB_RETURN_OK; gendb->done_forward = TRUE; return hard_link_ComplexSequence(gendb->forw); } /* is a seq db */ seq = init_SequenceDB(gendb->sdb,return_status); if( seq == NULL || *return_status == DB_RETURN_ERROR || *return_status == DB_RETURN_END ) { return NULL; /** error already reported **/ } /* check dna status. We assumme someone knows what he is doing when he makes a genomic db!*/ if( seq->type != SEQUENCE_DNA) { warn("Sequence from %s data entry doesn't look like DNA. Forcing it to",seq->name); } force_to_dna_Sequence(seq,1.0,NULL); /* map to Genomic on length of N buiness */ gendb->current = Genomic_from_Sequence_Nheuristic(seq,gendb->length_of_N); gendb->done_forward = TRUE; cs = evaluate_ComplexSequence_Genomic(gendb->current,gendb->cses,0,gendb->repeat_in_cds_score); if( cs == NULL ) { warn("Cannot make initial ComplexSequence. Unable to error catch this. Failing!"); *return_status = DB_RETURN_ERROR; return NULL; } return cs; } /* Function: reload_GenomicDB(last,gendb,return_status) * * Descrip: function which reloads the database * * * Arg: last [UNKN ] previous complex sequence, will be freed [ComplexSequence *] * Arg: gendb [UNKN ] Undocumented argument [GenomicDB *] * Arg: return_status [WRITE] return_status of the load [int *] * * Return [UNKN ] Undocumented return value [ComplexSequence *] * */ # line 205 "genomicdb.dy" ComplexSequence * reload_GenomicDB(ComplexSequence * last,GenomicDB * gendb,int * return_status) { ComplexSequence * cs; Sequence * seq; Genomic *temp; Genomic * gen; /** NB - notice that we don't do silly things with free's. Maybe we should **/ if( gendb->is_single_seq == TRUE ) { if( gendb->done_forward == TRUE ) { *return_status = DB_RETURN_OK; gendb->done_forward = FALSE; return hard_link_ComplexSequence(gendb->rev); } else { *return_status = DB_RETURN_END; return NULL; } } /** standard database **/ /** free Complex Sequence **/ if ( last != NULL ) { free_ComplexSequence(last); } if( gendb->done_forward == TRUE ) { if( gendb->current == NULL ) { warn("A bad internal genomic db error - unable to find current sequence in db reload"); *return_status = DB_RETURN_ERROR; return NULL; } temp = reverse_complement_Genomic(gendb->current); if( temp == NULL ) { warn("A bad internal genomic db error - unable to reverse complements current"); *return_status = DB_RETURN_ERROR; return NULL; } cs = evaluate_ComplexSequence_Genomic(temp,gendb->cses,0,gendb->repeat_in_cds_score); if( cs == NULL ) { warn("A bad internal genomic db error - unable to make complex sequence in db reload"); *return_status = DB_RETURN_ERROR; return NULL; } free_Genomic(temp); gendb->done_forward = FALSE; return cs; } /* otherwise we have to get a new sequence */ seq = reload_SequenceDB(NULL,gendb->sdb,return_status); if( seq == NULL || *return_status == DB_RETURN_ERROR || *return_status == DB_RETURN_END ) { return NULL; /** error already reported **/ } uppercase_Sequence(seq); /* check dna status. We assumme someone knows what he is doing when he makes a genomic db!*/ if( seq->type != SEQUENCE_DNA) { warn("Sequence from %s data entry doesn't look like DNA. Forcing it to",seq->name); } force_to_dna_Sequence(seq,1.0,NULL); if( force_to_dna_Sequence(seq,0.1,NULL) == FALSE ) { if( gendb->error_handling == GENDB_READ_THROUGH ) { warn("Unable to map %s sequence to a genomic sequence, but ignoring that for the moment...",seq->name); free_Sequence(seq); return reload_GenomicDB(NULL,gendb,return_status); } else { warn("Unable to map %s sequence to a genomic sequence. Failing",seq->name); *return_status = DB_RETURN_ERROR; return NULL; } } gen = Genomic_from_Sequence_Nheuristic(seq,gendb->length_of_N); cs = evaluate_ComplexSequence_Genomic(gen,gendb->cses,0,gendb->repeat_in_cds_score); if( cs == NULL ) { if( gendb->error_handling == GENDB_READ_THROUGH ) { warn("Unable to map %s sequence to a genomic sequence, but ignoring that for the moment...",seq->name); free_Sequence(seq); return reload_GenomicDB(NULL,gendb,return_status); } else { warn("Unable to map %s sequence to a genomic sequence. Failing",seq->name); *return_status = DB_RETURN_ERROR; return NULL; } } gendb->current = free_Genomic(gendb->current); gendb->current = gen; gendb->done_forward= TRUE; return cs; } /* Function: close_GenomicDB(cs,gendb) * * Descrip: top level function which closes the genomic database * * * Arg: cs [UNKN ] last complex sequence [ComplexSequence *] * Arg: gendb [UNKN ] protein database [GenomicDB *] * * Return [UNKN ] Undocumented return value [boolean] * */ # line 324 "genomicdb.dy" boolean close_GenomicDB(ComplexSequence * cs,GenomicDB * gendb) { if( gendb->is_single_seq == TRUE ) { return TRUE; } if( cs != NULL) free_ComplexSequence(cs); if( gendb->current != NULL) gendb->current = free_Genomic(gendb->current); return close_SequenceDB(NULL,gendb->sdb); } /* Function: new_GenomicDB_from_single_seq(gen,cses,score_in_repeat_coding) * * Descrip: To make a new genomic database * from a single Genomic Sequence with a eval system * * * Arg: gen [UNKN ] sequence which as placed into GenomicDB structure. [Genomic *] * Arg: cses [UNKN ] Undocumented argument [ComplexSequenceEvalSet *] * Arg: score_in_repeat_coding [UNKN ] Undocumented argument [int] * * Return [UNKN ] Undocumented return value [GenomicDB *] * */ # line 345 "genomicdb.dy" GenomicDB * new_GenomicDB_from_single_seq(Genomic * gen,ComplexSequenceEvalSet * cses,int score_in_repeat_coding) { ComplexSequence * cs,*cs_rev; GenomicDB * out; Genomic * temp; cs = evaluate_ComplexSequence_Genomic(gen,cses,0,score_in_repeat_coding); temp = reverse_complement_Genomic(gen); cs_rev = evaluate_ComplexSequence_Genomic(temp,cses,0,score_in_repeat_coding); out = new_GenomicDB_from_forrev_cseq(cs,cs_rev); out->single = hard_link_Genomic(gen); out->revsingle = temp; return out; } /* Function: new_GenomicDB_from_forrev_cseq(cs,cs_rev) * * Descrip: To make a new genomic database * from a single ComplexSequence * * * Arg: cs [UNKN ] complex sequence which is held. [ComplexSequence *] * Arg: cs_rev [UNKN ] Undocumented argument [ComplexSequence *] * * Return [UNKN ] Undocumented return value [GenomicDB *] * */ # line 369 "genomicdb.dy" GenomicDB * new_GenomicDB_from_forrev_cseq(ComplexSequence * cs,ComplexSequence * cs_rev) { GenomicDB * out; out = GenomicDB_alloc(); out->is_single_seq = TRUE; out->forw = cs; out->rev = cs_rev; return out; } /* Function: new_GenomicDB(seqdb,cses,length_of_N,repeat_in_cds_score) * * Descrip: To make a new genomic database * * * Arg: seqdb [UNKN ] sequence database [SequenceDB *] * Arg: cses [UNKN ] protein evaluation set [ComplexSequenceEvalSet *] * Arg: length_of_N [UNKN ] Undocumented argument [int] * Arg: repeat_in_cds_score [UNKN ] Undocumented argument [int] * * Return [UNKN ] Undocumented return value [GenomicDB *] * */ # line 390 "genomicdb.dy" GenomicDB * new_GenomicDB(SequenceDB * seqdb,ComplexSequenceEvalSet * cses,int length_of_N,int repeat_in_cds_score) { GenomicDB * out; if( seqdb == NULL || cses == NULL ) { warn("Attempting to make GenomicDB from some NULL objects."); return NULL; } /** should check sequence database **/ if( cses->type != SEQUENCE_GENOMIC ) { warn("You can't make a genomic database with a non SEQUENCE_GENOMIC cses type [%d]",cses->type); return NULL; } out = GenomicDB_alloc(); out->is_single_seq = FALSE; out->sdb = hard_link_SequenceDB(seqdb); out->cses = hard_link_ComplexSequenceEvalSet(cses); out->length_of_N = length_of_N; out->repeat_in_cds_score = repeat_in_cds_score; return out; } # line 403 "genomicdb.c" /* Function: hard_link_GenomicDB(obj) * * Descrip: Bumps up the reference count of the object * Meaning that multiple pointers can 'own' it * * * Arg: obj [UNKN ] Object to be hard linked [GenomicDB *] * * Return [UNKN ] Undocumented return value [GenomicDB *] * */ GenomicDB * hard_link_GenomicDB(GenomicDB * obj) { if( obj == NULL ) { warn("Trying to hard link to a GenomicDB object: passed a NULL object"); return NULL; } obj->dynamite_hard_link++; return obj; } /* Function: GenomicDB_alloc(void) * * Descrip: Allocates structure: assigns defaults if given * * * * Return [UNKN ] Undocumented return value [GenomicDB *] * */ GenomicDB * GenomicDB_alloc(void) { GenomicDB * out;/* out is exported at end of function */ /* call ckalloc and see if NULL */ if((out=(GenomicDB *) ckalloc (sizeof(GenomicDB))) == NULL) { warn("GenomicDB_alloc failed "); return NULL; /* calling function should respond! */ } out->dynamite_hard_link = 1; out->is_single_seq = FALSE; out->done_forward = FALSE; out->forw = NULL; out->rev = NULL; out->sdb = NULL; out->current = NULL; out->cses = NULL; out->error_handling = GENDB_READ_THROUGH; out->single = NULL; out->revsingle = NULL; out->length_of_N = 10; out->repeat_in_cds_score = -1000000; return out; } /* Function: free_GenomicDB(obj) * * Descrip: Free Function: removes the memory held by obj * Will chain up to owned members and clear all lists * * * Arg: obj [UNKN ] Object that is free'd [GenomicDB *] * * Return [UNKN ] Undocumented return value [GenomicDB *] * */ GenomicDB * free_GenomicDB(GenomicDB * obj) { if( obj == NULL) { warn("Attempting to free a NULL pointer to a GenomicDB obj. Should be trappable"); return NULL; } if( obj->dynamite_hard_link > 1) { obj->dynamite_hard_link--; return NULL; } if( obj->forw != NULL) free_ComplexSequence(obj->forw); if( obj->rev != NULL) free_ComplexSequence(obj->rev); if( obj->sdb != NULL) free_SequenceDB(obj->sdb); if( obj->current != NULL) free_Genomic(obj->current); if( obj->cses != NULL) free_ComplexSequenceEvalSet(obj->cses); if( obj->single != NULL) free_Genomic(obj->single); if( obj->revsingle != NULL) free_Genomic(obj->revsingle); ckfree(obj); return NULL; } /* Function: replace_is_single_seq_GenomicDB(obj,is_single_seq) * * Descrip: Replace member variable is_single_seq * For use principly by API functions * * * Arg: obj [UNKN ] Object holding the variable [GenomicDB *] * Arg: is_single_seq [OWNER] New value of the variable [boolean] * * Return [SOFT ] member variable is_single_seq [boolean] * */ boolean replace_is_single_seq_GenomicDB(GenomicDB * obj,boolean is_single_seq) { if( obj == NULL) { warn("In replacement function is_single_seq for object GenomicDB, got a NULL object"); return FALSE; } obj->is_single_seq = is_single_seq; return TRUE; } /* Function: access_is_single_seq_GenomicDB(obj) * * Descrip: Access member variable is_single_seq * For use principly by API functions * * * Arg: obj [UNKN ] Object holding the variable [GenomicDB *] * * Return [SOFT ] member variable is_single_seq [boolean] * */ boolean access_is_single_seq_GenomicDB(GenomicDB * obj) { if( obj == NULL) { warn("In accessor function is_single_seq for object GenomicDB, got a NULL object"); return FALSE; } return obj->is_single_seq; } /* Function: replace_done_forward_GenomicDB(obj,done_forward) * * Descrip: Replace member variable done_forward * For use principly by API functions * * * Arg: obj [UNKN ] Object holding the variable [GenomicDB *] * Arg: done_forward [OWNER] New value of the variable [boolean] * * Return [SOFT ] member variable done_forward [boolean] * */ boolean replace_done_forward_GenomicDB(GenomicDB * obj,boolean done_forward) { if( obj == NULL) { warn("In replacement function done_forward for object GenomicDB, got a NULL object"); return FALSE; } obj->done_forward = done_forward; return TRUE; } /* Function: access_done_forward_GenomicDB(obj) * * Descrip: Access member variable done_forward * For use principly by API functions * * * Arg: obj [UNKN ] Object holding the variable [GenomicDB *] * * Return [SOFT ] member variable done_forward [boolean] * */ boolean access_done_forward_GenomicDB(GenomicDB * obj) { if( obj == NULL) { warn("In accessor function done_forward for object GenomicDB, got a NULL object"); return FALSE; } return obj->done_forward; } /* Function: replace_forw_GenomicDB(obj,forw) * * Descrip: Replace member variable forw * For use principly by API functions * * * Arg: obj [UNKN ] Object holding the variable [GenomicDB *] * Arg: forw [OWNER] New value of the variable [ComplexSequence *] * * Return [SOFT ] member variable forw [boolean] * */ boolean replace_forw_GenomicDB(GenomicDB * obj,ComplexSequence * forw) { if( obj == NULL) { warn("In replacement function forw for object GenomicDB, got a NULL object"); return FALSE; } obj->forw = forw; return TRUE; } /* Function: access_forw_GenomicDB(obj) * * Descrip: Access member variable forw * For use principly by API functions * * * Arg: obj [UNKN ] Object holding the variable [GenomicDB *] * * Return [SOFT ] member variable forw [ComplexSequence *] * */ ComplexSequence * access_forw_GenomicDB(GenomicDB * obj) { if( obj == NULL) { warn("In accessor function forw for object GenomicDB, got a NULL object"); return NULL; } return obj->forw; } /* Function: replace_rev_GenomicDB(obj,rev) * * Descrip: Replace member variable rev * For use principly by API functions * * * Arg: obj [UNKN ] Object holding the variable [GenomicDB *] * Arg: rev [OWNER] New value of the variable [ComplexSequence *] * * Return [SOFT ] member variable rev [boolean] * */ boolean replace_rev_GenomicDB(GenomicDB * obj,ComplexSequence * rev) { if( obj == NULL) { warn("In replacement function rev for object GenomicDB, got a NULL object"); return FALSE; } obj->rev = rev; return TRUE; } /* Function: access_rev_GenomicDB(obj) * * Descrip: Access member variable rev * For use principly by API functions * * * Arg: obj [UNKN ] Object holding the variable [GenomicDB *] * * Return [SOFT ] member variable rev [ComplexSequence *] * */ ComplexSequence * access_rev_GenomicDB(GenomicDB * obj) { if( obj == NULL) { warn("In accessor function rev for object GenomicDB, got a NULL object"); return NULL; } return obj->rev; } /* Function: replace_sdb_GenomicDB(obj,sdb) * * Descrip: Replace member variable sdb * For use principly by API functions * * * Arg: obj [UNKN ] Object holding the variable [GenomicDB *] * Arg: sdb [OWNER] New value of the variable [SequenceDB *] * * Return [SOFT ] member variable sdb [boolean] * */ boolean replace_sdb_GenomicDB(GenomicDB * obj,SequenceDB * sdb) { if( obj == NULL) { warn("In replacement function sdb for object GenomicDB, got a NULL object"); return FALSE; } obj->sdb = sdb; return TRUE; } /* Function: access_sdb_GenomicDB(obj) * * Descrip: Access member variable sdb * For use principly by API functions * * * Arg: obj [UNKN ] Object holding the variable [GenomicDB *] * * Return [SOFT ] member variable sdb [SequenceDB *] * */ SequenceDB * access_sdb_GenomicDB(GenomicDB * obj) { if( obj == NULL) { warn("In accessor function sdb for object GenomicDB, got a NULL object"); return NULL; } return obj->sdb; } /* Function: replace_current_GenomicDB(obj,current) * * Descrip: Replace member variable current * For use principly by API functions * * * Arg: obj [UNKN ] Object holding the variable [GenomicDB *] * Arg: current [OWNER] New value of the variable [Genomic *] * * Return [SOFT ] member variable current [boolean] * */ boolean replace_current_GenomicDB(GenomicDB * obj,Genomic * current) { if( obj == NULL) { warn("In replacement function current for object GenomicDB, got a NULL object"); return FALSE; } obj->current = current; return TRUE; } /* Function: access_current_GenomicDB(obj) * * Descrip: Access member variable current * For use principly by API functions * * * Arg: obj [UNKN ] Object holding the variable [GenomicDB *] * * Return [SOFT ] member variable current [Genomic *] * */ Genomic * access_current_GenomicDB(GenomicDB * obj) { if( obj == NULL) { warn("In accessor function current for object GenomicDB, got a NULL object"); return NULL; } return obj->current; } /* Function: replace_cses_GenomicDB(obj,cses) * * Descrip: Replace member variable cses * For use principly by API functions * * * Arg: obj [UNKN ] Object holding the variable [GenomicDB *] * Arg: cses [OWNER] New value of the variable [ComplexSequenceEvalSet *] * * Return [SOFT ] member variable cses [boolean] * */ boolean replace_cses_GenomicDB(GenomicDB * obj,ComplexSequenceEvalSet * cses) { if( obj == NULL) { warn("In replacement function cses for object GenomicDB, got a NULL object"); return FALSE; } obj->cses = cses; return TRUE; } /* Function: access_cses_GenomicDB(obj) * * Descrip: Access member variable cses * For use principly by API functions * * * Arg: obj [UNKN ] Object holding the variable [GenomicDB *] * * Return [SOFT ] member variable cses [ComplexSequenceEvalSet *] * */ ComplexSequenceEvalSet * access_cses_GenomicDB(GenomicDB * obj) { if( obj == NULL) { warn("In accessor function cses for object GenomicDB, got a NULL object"); return NULL; } return obj->cses; } #ifdef _cplusplus } #endif